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Ligase: Sticking DNA Together
Ligase: Sticking DNA Together
Ligase is the enzyme used in genetic engineering to join sections of DNA together.
Genetically engineering bacteria to produce a useful substance has a simple set of steps. What are the first and last steps in the process?
You can select multiple answers
Which of these DNA sequences could be joined to this sequence? AAGCTT
Restriction enzymes create ends that can later be joined together, but the ends on the gene and the plasmid must match each other.
Which end would match with the sticky end of this gene shown above?
Fill in the gap: ****
Ligase enzymes join open ends of DNA strands together. They have the opposite function to a ___________ enzyme.
DNA is made from a backbone of sugar and phosphate molecules supporting a chain of bases. The sugar-phosphate bonds hold the backbone of the DNA together. Which chemicals make up the DNA code for a protein?
Which bonds would have to be broken to cut open the DNA and pull apart the backbone ?
A) Sugar-phosphate bond. B) Sugar-base bond. C) Base-Base bond.
The diagram shows only the cut end of the plasmid DNA at the top, and the cut DNA of the gene to be added to it at the bottom. The rest of the two DNA strands are not shown in the diagram.
The base pairs of the two cut ends match, so it will make a valid join.
To join the two ends of the DNA together, the phosphate-sugar bonds have to be reformed to hold the backbone of the DNA together. Which of these enzymes is used for this? Restriction or ligase?
It requires energy to reform this phosphate-sugar bond. What do we call the molecule in cells that provides the energy for this reaction?